Amino acid composition and protein quality of instant liquid food based on Catfish (Clarias gariepinus) and Kelor (Moringa oleifera) flour

Abstract

Under-five children are a vulnerable group whose susceptible to repeated infections and deficiency of protein and calcium. The objective of this research is to analyze the amino acid composition and protein quality of instant liquid food based on catfish (Clarias gariepinus) and kelor (Moringa oleifera) flour according to the requirement of children 1-3 years old. The amino acid composition was analyzed by using Ultra Performance Liquid Chromatography (UPLC). The highest essential amino acid content in the instant liquid food based on catfish and kelor flour is the 114.29±0.79 mg/g protein lysine and the highest non-essential amino acid is the glutamic acid of 244.14±1.86 mg/g protein. The protein quality was determined by the chemical score (78.30 for sulphuric amino acid), the amino acid score (100), the essential amino acid index (EAAI = 0.98), the predicted protein efficiency ratio (P-PER = 4.63±0.02), and the predicted biological value (P-BV = 48.51±0.13).The study showed that instant liquid food based catfish and kelor flour containing high-quality protein and calcium that is recommended for children aged 1-3 years.