Abstract

Contact through protoplasmic extensions from the protoplasts was infrequently observed and cell–cell contact involving large surface areas was not observed when adult Periplaneta americana hemocytes were incubated with Entomophaga aulicae protoplasts in in vitro culture for up to 30 min. Such contact did not appear to alter the subsequent morphology of either the protoplast or the hemocyte. Coagulocyte, plasmatocyte, and granulocyte aggregates along with individual coagulocytes and granulocytes produced a melaninlike material upon incubation with protoplasts. Incubation of the hemocytes in the presence of protoplasts did not alter the affinity of hemocytes for Sephadex beads. The results of this study indicate that there was no detectable cell-mediated immune reaction of cockroach hemocytes to E. aulicae protoplasts. The role of the melaninlike material is unknown.

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