Ameliorative Role of Vitamin E on Adverse Impact of Azadirachta indica in Reproductive Indices of Male Rats

Abstract

This study was undertaken to determine the role of vitamin E on reproductive activity of Azadirachta indica extract in rats. Forty adult male albino rats were divided into 5 equal groups. The control group received distilled water while the other groups were treated with Azadirachta indica (150mg/kg bwt) extract alone, vitamin E (100mg/kg bwt) alone, combined Azadirachta indica (150mg/kg bwt) and vitamin E (100mg/kg bwt), and combined Azadirachta indica (150 mg/kg bwt) and vitamin E (150 mg/kg bwt). Group administered Azadirachta indica alone showed significant (p<0.05) decrease in sperm count, viability and motility. Co-administration of A. indica with vitamin E caused an increase in values of these parameters. Serum testosterone concentration decreased significantly (p<0.05) in A. indica only treated group with concomitant increase in groups co-administered with vitamin E. Lipid peroxidation showed significant decrease (p<0.05) in all treated groups with group co-administered with vitamin E showing enhanced modulatory effect than group administered with Azadirachta indica extract alone. Superoxide dismutase (SOD) activity was also significantly (p<0.05) enhanced in all treated groups; the groups treated with vitamin E showed higher activity. The results suggest possible ameliorative effect of vitamin E on adverse impact of Azadirachta indica in reproductive activity of male rats.