Abstract
Aim: The present investigation has been conducted to evaluate the protective activity of Tephrosia purpurea extract (TPE) against arsenic induced toxicity. Materials and Methods: For this study, twenty four wistar albino rats were taken. Control group, group – I rats were given sodium arsenite @ 10 mg/kg and group – II rats were treated with TPE @ 500 mg/kg along with sodium arsenite @ 10 mg/kg (daily oral for 28 days). On 29th day animals were slaughtered and various parameters were determined. Serum biomarkers, haematological parameter analysis and histomorphological examination are carried out with estimation of arsenic concentration in tissues. Results: Oral administration of sodium arsenite @ 10 mg/kg for 28 days resulted in a significant decrease in Hb%, TEC and TLC, significant increase of serum glucose, cholesterol, calcium and significant increase in arsenic accumulation in tissues. Histopathological results of intestine revealed haemorrhagic enteritis along with loss of villi. Treatment with Tephrosia purpurea @ 500 mg/kg significantly decreased the elevated glucose, LDH levels, along with significant increase haematological levels towards normal. There was reduced haemorrhagic enteritis and presence of intact villi, as compared to arsenic treated group. But there was no significant difference in serum calcium, serum cholesterol and arsenic concentration in tissues, when compared with arsenic treated group. Conclusion: The study conclude that supplementation of TPE (500 mg/kg) daily oral for 28 days has shown protection against arsenic induced toxicity by its protective effect.
Highlights
Arsenic is a toxic metalloid and is a common environmental pollutant, virtually present in rocks, soil and water and is second most important pollutant next to lead [1]
Oral administration of sodium arsenite @ 10 mg/kg for 28 days resulted in a significant decrease in Hb%, Total erythrocyte count (TEC) and Total leucocyte count (TLC), significant increase of serum glucose, cholesterol, calcium and significant increase in arsenic accumulation in tissues
There was no significant difference in serum calcium, serum cholesterol and arsenic concentration in tissues, when compared with arsenic treated group
Summary
Arsenic is a toxic metalloid and is a common environmental pollutant, virtually present in rocks, soil and water and is second most important pollutant next to lead [1]. Exposure to arsenic is known to induce a wide range of toxic effects on brain [2], induces fibrosis of liver [3] and oxidative stress in the body. Arsenic mainly effects liver and kidney [4] and causes damage to them. Herbal plants with flavonoids and polyphenols are potent inhibitors of oxidative stress [5]. Tephrosia purpurea is commonly known as “Sharphunka” in Hindi and its leaves were used as constituent in liver tonics [6], have a potent nephroprotective activity [7] and antioxidant property [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
