Ameliorative Potential of Moringa Seed Oil Extract on Chlorambucil-Induced Liver Injury in Adult Male Albino Rat: Biochemical, Histological and Immunohistochemical studies

Abstract

Chlorambucil is similar to the other chemotherapeutic drugs that induce direct hepatotoxicity. The present work aimed to investigate the protective role of moringa oil against chlorambucil- induced liver toxicity in rats. In this study, adult male albino rats were equally divided into four main groups: Group I: control rats fed daily with standard food diet and free access of water; group II: rats subjected to force feeding and received moringa oil (2 ml Kg b.w day) for four weeks; group III: rats received orally chlorambucil (1.26 mg Kg b.w day) for four weeks; group IV: rats received orally chlorambucil and moringa oil with same previous doses for four weeks. Thereafter, rats were anesthetized and blood samples were collected to separate sera to determine the activities of both alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) as well as the level of total bilirubin. In addition, liver specimens were prepared for both histopathological and immunohistochemical examinations. The present results showed that chlorambucil caused significant increase in the activities of both alanine and aspartate aminotransferases and the level of total bilirubin. In addition, massive histopathological and immunohistochemical changes were observed in the liver sections as compared to the control group. On the other hand, with giving moringa oil together with chlorambucil resulted in a significant decrease in the activities of both alanine and aspartate aminotransferases and the level of total bilirubin as well as marked apparent improvement as regards the histopathological and the immunohistochemical changes, comparing to those of chlorambucil group. In conculsion, Moringa oil significantly reversed chlorambucil-induced hepatotoxicity in albino rats.