Abstract
The aim of this study was to compare between the effect of minocycline or lactulose alone and in combination on experimentally-induced colitis in rats.Materials and methods:Seventy albino rats were divided into 7 equal groups: Control untreated group, trinitrobenzenesulphonic acid (TNBS) group, TNBS + lactulose group, lactulose group, TNBS + minocycline group, minocycline group and TNBS + lactulose+ minocycline group. A part of the colon was homogenized for determination of tissue tumor necrosis factor alpha, malondialdehyde, nitric oxide, reduced glutathione and myeloperoxidase. The other part of the colon was examined histopathologically. Also, disease activity index was measured.Results:Administration of each of lactulose or minocycline alone and in combination to TNBS-treated rats induced significant increase in tissue reduced glutathione with significant decrease in disease activity index, tissue tumor necrosis factor alpha, malondialdehyde, nitric oxide and myeloperoxidase and alleviated the histopathological changes compared to the group that received TNBS alone. Minocycline/lactulose combination produced significant improvement in the biochemical and histopathological parameters compared to the groups that received either minocycline or lactulose alone.Conclusion:Lactulose and minocycline had protective effects on TNBS-induced colitis in rats but minocycline/lactulose combination had the upper hand.
Highlights
Colitis is a form of inflammatory bowel diseases that includes characteristic ulcers or open sores
Administration of each of lactulose or minocycline to normal rats produced non-significant effect on disease activity index (DAI) compared to the control untreated group
The decrease in DAI was significant in minocycline/lactulose combination group compared to the use of either minocycline or lactulose alone (Fig.1)
Summary
Colitis is a form of inflammatory bowel diseases that includes characteristic ulcers or open sores. The symptoms of colitis can sometimes diminish spontaneously, the disease usually requires treatment to go into remission. It is thought to be an antinflammatory drug that is essentially providing topical relief inside the intestine It has many adverse effects which necessitate searching for alternative and adjuvant agents [8]. Non-digestible sugar used in treatment of chronic constipation and hepatic encephalopathy. It is a disaccharide formed of fructose and galactose. Oral administration of lactulose significantly increased H2 production which may has antioxidant and anti-inflammatory properties in the gastrointestinal tract [11, 12]. Minocycline was reported to have antioxidant and antiapoptotic properties that may provide beneficial effects in the treatment of colitis [15]. The aim of this study was to compare between the effect of minocycline or lactulose alone and in combination on experimentally-induced colitis in rats
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