Abstract
Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H2O2)-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss.
Highlights
Bone remodeling is a dynamic and continuous process that maintains bone health through a tight coupling between the activity of bone-forming osteoblasts and bone-resorbing osteoclasts (Drissi & Sanjay, 2016). An imbalance in this remodeling process may lead to weak bone formation which results in osteoporosis, one of the most common skeletal diseases characterized by reduced bone mineral density (BMD) and deteriorated bone microstructure (Cauley, 2017)
We evaluated the effects of P-Antarctic krill peptide (AKP) as an antioxidant agent, as well as a protective agent on H2O2-induced oxidative injury model in MC3T3-E1 cells
The present study showed that the antioxidant activity of phosphorylated Antarctic krill peptide (P-AKP) was enhanced by the introduction of additional phosphates by dry-heating method
Summary
Bone remodeling is a dynamic and continuous process that maintains bone health through a tight coupling between the activity of bone-forming osteoblasts and bone-resorbing osteoclasts (Drissi & Sanjay, 2016). An imbalance in this remodeling process may lead to weak bone formation which results in osteoporosis, one of the most common skeletal diseases characterized by reduced bone mineral density (BMD) and deteriorated bone microstructure (Cauley, 2017). Due to the increased life expectancy, osteoporosis has become a severe socio-economic issue and a major public health problem (Odén et al, 2015; Sözen et al, 2017). As side effects of current therapeutic agents have been reported, some alternative protective and preventive methods are highly desirable, especially the use of safe bioactive substances with osteogenic effects (Huang et al, 2015)
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