Ameliorative effects of dietary Vitamin‐C on growth performance and hemato‐biochemical response of sodium fluoride‐intoxicated Amur Carp, Cyprinus carpio haematopterus

Abstract

This study aimed to assess the ameliorative dietary effect of vitamin-C against sodium fluoride (NaF)-induced toxicity on growth and haemato-biochemical parameters in Amur carp. A total of 150 fish (average body weight 169 ± 0.69 g) were randomly distributed into five groups (with three triplicates) including G1 (control); G2 (NaF exposure @100 ppm); G3 (vitamin-C supplemented diet @100 ppm); G4 (both NaF exposure @100 ppm and vitamin-C supplemented diet @100 ppm) and G5 (NaF exposure @100 ppm for 60 days and then vitamin-C supplemented diet @200 ppm for last 30 days). Results revealed that growth and hemato-biochemical profile was best in G3 in comparison with G1, whereas groups G2, G4 and G5 have shown retarded growth, a decrease in Hb, TEC, PCV, MCV, MCH and MCHC. Serum-biochemical parameters were found optimum in G3 as compared with G1 and in groups of G2, G4 and G5, total serum protein, albumin, globulin, glucose, and triglyceride levels decreased significantly, whereas creatinine, AST and ALT levels increased significantly. In G4, toxicity was less severe in comparison with G2, whereas in G5, after the dietary supplementary of vitamin-C, parameters have shown a bend towards the normal values. Thus, it can be concluded that vitamin-C has ameliorative action against fluoride toxicity in fish.