Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats.

Tugba Yilmaz-Ozden,Refiye Yanardag,Ozlem Kurt-Sirin,Sevim Tunali,Nuriye Akev,Ayse Can

doi:10.17305/bjbms.2014.2273

Tugba Yilmaz-Ozden, Refiye Yanardag + Show 4 more

Open Access

https://doi.org/10.17305/bjbms.2014.2273

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Abstract

Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), myeloperoxidase (MPO), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD) and lactate dehydrogenase (LDH) activities were determined in the stomach tissue. CAT, SOD, GR, GPx, GST, CA, G6PD and LDH activities were increased in diabetic rats when compared to normal rats. Vanadium treatment significantly reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G6PD and LDH activities were insignificant. No significant change was seen for MPO activity between the groups. It was concluded that vanadium could be used for its ameliorative effect against oxidative stress in diabetes.

Highlights

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and insufficency of secretion or action of endogenous insulin
superoxide dismutase (SOD) (p < . ), glutathione reductase (GR) (p < . ), glutathione peroxidase (GPx) (p < . ), GST (p < . ), carbonic anhydrase (CA) (p < . ), glucose- -phosphate dehydrogenase (G PD) (p < . ) and lactate dehydrogenase (LDH) (p < . ) activities significantly but CAT activity insignificantly increased in stomach tissue when compared to the control group
Vanadium supplementation to the diabetic rats significantly (p < . ) reduced the elevated activities of GR, GPx, GST compared with the diabetic group whereas the decreases in CAT, SOD, CA, G PD and LDH activities were insignificant

Summary

Introduction

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and insufficency of secretion or action of endogenous insulin. While exogenous insulin and other medications can control many aspects of diabetes, numerous complications affecting several tissues are common and are extremely costly in terms of longevity and quality of life [ ]. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of both types of diabetes mellitus. The consequences of oxidative stress can promote the development of complications of diabetes mellitus [ ]. Despite numerous studies during the past decade, the mechanism(s) by which vanadium mediates its in vivo antidiabetic effects are not well understood [ ]. Smith et al [ ] states that there is no rigorous evidence that oral vanadium supplementation improves glycaemic control in type diabetes and that the routine use of vanadium for this purpose cannot be recomended.

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