Ameliorative effect of Tinospora cordifolia stem aqueous extract on Hematological parameters of wistar rat against HgCl2 induced toxicity

Abstract

Blood is crucial for the human body's health and survival. It carries out a variety of functions, including as delivering nutrients and oxygen. RBCs, WBCs, plasma, and platelets are the blood's major four constituents. It serves as a pathological sign for the condition of animals under different situations and toxicant exposure. This work aimed to explore the in vivo therapeutic mechanism of Tinospora cordifolia aqueous extracts against the haematotoxic effects of HgCl2. Ameliorative effect of T.cordifolia aqueous extract was evaluated in two groups of rats, including a treated group and a control group. The treated group was divided into following subgroups, I: HgCl2 treated, subgroup II: HgCl2 + T. cordifolia treated, subgroup III: T. cordifolia treated. These treated subgroups were further divided into 5 set of rats, one for acute (1d), three for sub-acute (7, 14 and 28ds), and one for sub-chronic (60 ds) study. The controls were run simultaneously. Male Wistar rats were given aqueous extracts of T. cordifolia (400 mg/kg body weight, orally, once daily) along with HgCl2 treatments. Dose of HgCl2 administered to animals after calculation of LD50 (25 mg/kg body weight). Animals used in acute, sub-acute, and sub-chronic studies of mercuric chloride received 1/10, 1/50, and 1/100 of the calculated LD50. This corresponds to 2.5, 0.5 and 0.25 mg/kg respectively. In mercury-treated mice, significant reductions in WBC, RBC, Hb, PCV and platelet count were also observed. These HgCl2-related effects were circumvented by regular intake of T. cordifolia aqueous extract. These results show that T. cordifolia protects against HgCl2 poisoning.