AMELIORATIVE EFFECT OF SODIUM VALPROATE IN COMBINATION WITH JUGLANS REGIA FRUIT EXTRACT AGAINST PENTYLENETETRAZOLE INDUCED CONVULSIONS IN MICE

Abstract

Epilepsy is a complex neurological disorder characterized by hypersecretion of excitatory neurotransmitters in the brain. Level ofantioxidant enzymes in the brain changes during seizure. There are many herbal supplements that posses antioxidant potential and might help inprevention and recovery of seizures when used as an adjunct with modern antiepileptic drugs. Objective: The present work was undertaken toevaluate the Ameliorative effect of Sodium valproate in combination with Juglans regia extract against Pentylenetetrazole (PTZ) inducedconvulsions in mice. Materials and Methods: Juglans regia ethanolic extract (JREE) were administered at doses of (200mg/kg and 400 mg/kgp.o) and Sodium Valproate (500mg/kg p.o.) one hour before the administration of PTZ (80mg/kg i.p.). Brain tissues were screened for MDA,Catalase, GSH and protein estimation. Result and Discussion: Administration of JREE in the low and high dose prolongs the onset of myoclonicjerks dose-dependently and also showed protection against PTZ-induced convulsions. PTZ induced seizures caused a significant increase in MDAlevels and a significant decrease in GSH and catalase levels in PTZ group as compared to the vehicle control group. Juglans regia pre-treatmentprevented the oxidative stress as indicated by significant decrease in MDA levels and significant increase in GSH and catalse levels incomparison to PTZ group. Conclusion: The present study showed ameliorative effect of combination of Sodium Valproate in anticonvulsantactivity of ethanolic extract of Juglans regia fruit against PTZ induced seizures and also its protective effect against seizure induced oxidativestress.