Ameliorative Effect of Salicin Against Gamma Irradiation Induced Electrophoretic Changes in Brain Tissue in Male Rats

Abstract

The aim of this study was to investigate the radioprotective effect of salicin against irradiation effect on brain tissue of male rats. Lipid peroxidation level was measured as thiobarbituric acid reactive substance in brain tissue. The polyacrylamide gel electrophoresis for native protein, lipoprotein and zymogram were carried out in brain homogenate. As expected, salicin resisted the irradiation effect and declined the MDA level in brain homogenate of all treated groups (especially in the irradiated salicin post-treated group). Salicin minimized the qualitative mutagenic effect of irradiation on the electrophoretic protein pattern in all irradiated salicin treated groups and it showed the highest antagonistic effect against irradiation in irradiated salicin post-treated group (SI = 0.57). It could not prevent the abnormalities occurred qualitatively and quantitatively as a result of irradiation in lipoprotein pattern in all irradiated salicin treated groups. In the electrophoretic esterase pattern, salicin prevented the qualitative effect of irradiation in irradiated salicin post-treated group (SI = 1.00). Salicin minimized the qualitative irradiation effect on the catalase pattern in the irradiated salicin pre-treated group (SI = 0.73). While in the peroxidase pattern, salicin adminstration resisted the irradiation effect in the irrradiated post-treated groups (SI = 0.67). The results suggested the radioprotective ability of salicin against gamma irradiation effect on various electrophoretic patterns in brain tissue of male rats.