Ameliorative effect of novel nanocomposite: basic curcumin nanoparticles modified with basic nano black seeds (nigella sativa) and calcium ascorbate on metabolic changes in experimentally induced tumor in female mice

Abstract

The ameliorative effect and antitumor activity of basic curcumin nanoparticles modified with basic nanoblack seeds (nigella sativa) and calcium ascorbate were evaluated. eighty female mice were divided into4 equal groups of 20 mice each. Group 1: non – tumor bearing mice (NTBM). Group 2: tumor bearingmice(TBM). Group 3: NTBM + nanocomposite (4.750gm / kg. b.wt /day ) , orally for 6 weeks . Group4: TBM + nanocomposite (4.750gm / kg. b.wt/day ), orally for 6 weeks . Blood samples for serumseparation and liver tissues specimens were collected from all animal groups three times after 2, 4 and6 weeks from the onset of treatment. All serum was directly used for determination of ALT and ASTactivities and urea and creatinine concentrations. Moreover, antioxidants enzymes (catalase, GPX,SOD), GSH and MDA were detected in liver tissue. The obtained results showed significant decreasein catalase, GPX and SOD activities and GSH concentration in liver tissue of tumor bearing mice.Meanwhile, liver MDA, serum transferases(ALT and AST) activities, urea and creatinine concentrationswere significantly increased in tumor bearing mice . TBM treated with nanocomposite improve theadverse biochemical changes induced by tumor. So, this nanocomposite has potential benefits in cancertreatment