Ameliorative effect of morin on dutasteride-tamsulosin-induced testicular oxidative stress in rat.

Abstract

Dutasteride-Tamsulosin (DUT-TAM), a drug of choice for the treatment of prostate enlargement (Benign Prostatic Hyperplasia, BPH) has been implicated in testicular toxicity. This study investigated the protective effect ofmorin, a plant-derived flavonoid on DUT-TAM-induced testicular toxicity in Wistar rat. Twenty-four male Wistar rats (110-140g) were randomly divided into four treatment groups (n=6). Group A animals served as the control and were administered olive oil, Group B animals were administered 5.4mg/kg b.w. of dutasteride + 3.4mg/kg b.w of tamsulosin., Group C animals were administered 100mg/kg b.w. of morin, while Group D animals were administered DUT-TAM (5.4mg/kg b.w. of dutasteride + 3.4mg/kg b.w. of tamsulosin) and morin (100mg/kg b.w.). The administration lasted for two weeks. DUT-TAM-induced abnormal sperm morphology (31.8%), significantly reduced (p<0.05) sperm count, sperm motility, live-dead sperm ratio, testicular superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST) and acid phosphatase (ACP) activities, as well as the levels of ascorbic acid and reduced glutathione (GSH) which were ameliorated by co-treatment with morin. Also, DUT-TAM-induced increase in testicular malondialdehyde level and the activities of alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT) and lactate dehydrogenase (LDH) were significantly reversed (p<0.05) by co-treatment with morin. These results indicated the protective ability of morin against Dutasteride-Tamsulosin-induced testicular toxicity and oxidative stress.