Abstract
Fertility loss, recurrent spontaneous abortion and poor outcome in assisted reproductive techniques (ART) have been associated with DNA fragmentation. This work was achieved to evaluate the protective role of melatonin versus apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa from men with asthenoteratozoospermia (ATS). Some researchers maintain that melatonin can serve as a remedy for apoptosis induction, and it has an impressive effect on boosting the quality and quantity of spermatozoa. For this purpose, semen samples were collected from 50 ATS men and they were divided into control and melatonin (6mM) groups at 2, 4, 6 and 24hr. Concentrating on the reasons for apoptosis is an arduous process, but in the present study for this evaluation mitochondrial membrane potential (MMP), DNA fragmentation by TUNEL and sperm chromatin dispersion (SCD) methods and lipid peroxidation were carried out. Also, sperm viability was performed. In the control group, MDA, TUNEL-positive and SCD were significantly increased but viability and MMP of spermatozoa were significantly decreased. Moreover, in the melatonin group, TUNEL-positive, SCD and MDA levels were significantly decreased and viability and MMP significantly increased, compared to the control group. In outcome, melatonin prescription paves the way for apoptosis down-regulating in the ATS men.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
