Abstract
Photocoagulation is routinely used as a major treatment method for many retinal disorders. The present study was conducted to investigate the protective effect of Grape seed extract (GSE) on reactive oxygen species produced during argon laser photocoagulation and also to explore the benefit of retinal photocoagulation over two session's argon laser compared to single session. Forty two pigmented rabbits weighted 2–2.5 Kg were used in this study. The animals were classified into three groups. The left eye for each animal was photocoagulated with 200 mW, 400 mW and 400 mw fractionated dose (FD) argon laser with and without GSE supplementation respectively. After 24 hours the retina was separated carefully, malondialdehyde level (MDA), total antioxidant capacity (TAC) and Fourier transform infrared spectroscopy (FT-IR) for retinal tissue were detected. FT-IR findings showed a positive result for GSE supplementation in reducing laser effects on the retinal tissue. Also, GSE supplementation improved the level of TAC in rabbit's retina exposed to argon laser with a concurrent decrease in MDA level. In conclusion, GSE has an extremely beneficial role in overcoming the resultant adverse biological effects of argon laser photocoagulation on retinal tissues due to its potent antioxidant properties. Results also revealed that retinal photocoagulation over two sessions was more protective for retinal tissue than single session argon laser.
Highlights
Photocoagulation is used as a major treatment method for many retinal disorders, but it causes visible laser scars that can enlarge postoperatively and leads to many complications such as choroidal neovascularization and subretinal fibrosis Birnbach et al, 1994 [4]
The measurements were carried out on control rabbits, rabbits supplemented with Grape seed proanthocyanidins extract (GSPE) for one week before argon laser photocoagulation, and rabbits subjected to argon laser photocoagulation without GSPE supplementation
The recorded increase in malondialdehyde level (MDA) level in retinal tissue after treatment with argon laser indicated that laser cause oxidative stress to retinal tissue and this stress increased by increasing argon laser dose
Summary
Photocoagulation is used as a major treatment method for many retinal disorders, but it causes visible laser scars that can enlarge postoperatively and leads to many complications such as choroidal neovascularization and subretinal fibrosis Birnbach et al, 1994 [4]. Laser-induced injuries mainly include necrosis in the area caused by laser irradiation and apoptosis in the adjacent area due to a thermal effect. Many visual problems appear because injuries can occur in the adjacent normal tissue, such as retinal scarring, visual field defects and night vision loss Rui et al, 2014 [18]. “Ameliorative Effect of Grape Seed Proanthocyanidin Extract on Argon-Laser Induced Retinal Damage,” The Open Access Journal of Science and Technology, vol 5, Article ID 101307, 13 pages, 2017. “Ameliorative Effect of Grape Seed Proanthocyanidin Extract on Argon-Laser Induced Retinal Damage,” The Open Access Journal of Science and Technology, vol 5, Article ID 101307, 13 pages, 2017. doi:10.11131/2017/101307
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
