Ameliorative Effect of Different Concentration of Mushroom (&lt;i&gt;Pleurotus Tuberregium&lt;/i&gt;) on Pathomorphological Changes Induced By Lead Toxicity in Liver and Kidney of Wistar Albino Rats

Bj Enagbonma,O Osemwegie,P Tawari-Fufenyi,S Ekaye

doi:10.4314/njb.v29i1.9

Abstract

This study evaluates ameliorative effect of different concentrations of mushroom (Pleurotus tuberregium) on the effects of lead (Pb). Ninety albino rats were divided into six groups with three replicates (5 rats per group) for 21 days pre-experimental stage for acclimatization. The experimental groups were exposed to 0.1 g/l of lead daily for 21 days. At the end of exposure period, lead salt was discontinued for 21 and 42days to verify possible ameliorative effect of mushroom in the post-experimental stage. Samples of liver and kidney tissues were then collected and subsequently analyzed for structural changes every 21 days. The tissues of the pre-experimental stages showed normal tissues as those of the control except in the liver which showed mild periportal chronic inflammatory cell. However, the experimental groups, showed liver with focal periportal vascular congestion and hypertrophy, moderate chronic inflammatory cell infiltration and interstitial oedema. The kidney showed focal proximal tubular necrosis, transmural oedema, interstitial haemorrhage, and chronic inflammatory cell infiltration. The tissues of liver and kidney in the post experimental stages were improved with increased mushroom content in the feed. Results of this study demonstrated that P. tuberregium may ameliorate lead toxicity in liver and kidney tissues.Keywords: Abatement, Albino rat, Kidney and Liver, Lead toxicity, Pathomorphological Pleurotus tuberregium,

Highlights

Pathomorphological changes induced by lead acetate toxicity in wistar rats has been reported
This study attempts to investigate the ameliorative effect of different concentration of mushroom (Pleurotus tuberregium) on pathomorphological changes induced by lead toxicity in kidney and liver of wistar albino rats
When 0.1 g/l Pb was added to experimental diet sectioned kidney showed focal proximal tubular necrosis (A), transmural oedema (B), vascular hypertrophy and congestion (C), and interstitial haemorrhage (D) as represented in Plate 7 below.The sectioning of kidney of rat fed with marsh and P. tuberregium diet (50:50%), and treated with 0.1g/l Pb, showed slightly abated kidney with normal glomerulus (A), proximal convoluted tubules (B) and interstitial haemorrhage (C) (Plate 8)

Summary

Introduction

Pathomorphological changes induced by lead acetate toxicity in wistar rats has been reported. There has been an increase of interest in the therapeutic potential of medicinal plants as antioxidants in reducing free radical- induced tissue damage (Siddique et al, 2000 and Koleva et al, 2002). Pleurotus tuberregium is a tropical sclerotial mushroom that produces a sclerotium or an underground tuber as well as a fruiting body (sporocarp). Both the sclerotium and the mushroom are edible (Jin-zhong et al, 2003). This study attempts to investigate the ameliorative effect of different concentration of mushroom (Pleurotus tuberregium) on pathomorphological changes induced by lead toxicity in kidney and liver of wistar albino rats

Materials and Methods

Findings

Discussion