Ameliorative effect of baicalein nanoparticles on body weight loss and tumour growth in Daltons Lymphoma Ascites (DLA) cell induced tumour mice model

Abstract

Objective: Baicalein has demonstrated a wide variety of biological activities including anticancer property. The present study was aimed to evaluate effect of silver nanoparticles synthesised using baicalein on body weight and tumour weight in Daltons Lymphoma Ascites (DLA) cell induced solid tumour in Swiss albino mice. Methods: Tumour positive animals were randomly allocated to seven groups of six animals. Group I served as normal control Group II as tumour control, Group III was administered with 5-Fluorouracil (5-FU) at 20 mg/kg orally. Group IV and V received baicalein nanoparticles (B-AgNPs) at 50 mg/kg and 100 mg/kg respectively. Group VI received pure compound, baicalein (BCLN) at 100 mg/kg. Chemically synthesised nanoparticles (C-AgNPs) at 100 mg/kg was administered to Group VII. All the treatments were given orally for 10 days. Body weight was recorded on days zero, five and 11. All the animals were sacrificed after 10 days treatment and collected tumour mass to assess tumour weight and ratio of tumour weight to body weight. Result: All the groups except normal control showed reduction in body weight but the weight loss was significantly less compared to C-AgNPs treated group and tumour control group. A significant dose dependant reduction in tumour weight and ratio of body weight to tumour was noticed in animals treated with B-AgNPs at both high and low doses compared to other groups. Conclusion: The oral administration of B-AgNPs @100 mg/kg was found to have a significant ameliorative effect on body weight loss and tumour growth in Daltons Lymphoma Ascites (DLA) cell induced tumour mice model. In the present study it was also found that biosynthesised nanoparticles (B-AgNPs) produced more activity than the pure compound (BCLN) from which they were synthesised.