Abstract
BackgroundWe examined possible protective effect of Satureja khozestanica essential oil (SKE) on in vivo and in vitro lipid peroxidation in alloxan-induced Type 1 diabetic rats.MethodsThirty Sprage-dawley male rats were divided into three groups randomly; group one as control, group two diabetic untreatment, and group three treatments with SKE by 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, livers and kidneys were then removed immediately and used fresh or kept frozen until their lipid peroxidation analysis. Lipid peroxidation was determined by measurement of thiobarbituric acid reactive substances (TBARS). Blood samples were also collected before killing to measure the levels of fasting blood suger (FBS) and lipid peroxidation.ResultsSKE significantly inhibited the levels of FBS, TBARS serum and kidney content in treated group compared with the diabetic untreated group. Also the levels of malonedialdehyde liver content unaltered in treated group. SKE significantly inhibited LDL oxidation in vitro.ConclusionsThe findings showed that SKE exerts beneficial effects on the lipid peroxidation in alloxan-induced Type 1 diabetic rats.
Highlights
We examined possible protective effect of Satureja khozestanica essential oil (SKE) on in vivo and in vitro lipid peroxidation in alloxan-induced Type 1 diabetic rats
The components of Satureja khozestanica essential oil were analyzed with gas chromatography/mass spectrometry (GC/MS) in Research Center of Lorestan University (Table 1)
The level of thiobarbituric acid reactive substances (TBARS) in the serum of diabetic rats treated with SKE was very low, similar to the level found in the control animals
Summary
We examined possible protective effect of Satureja khozestanica essential oil (SKE) on in vivo and in vitro lipid peroxidation in alloxan-induced Type 1 diabetic rats. Oxidative stress is the imbalance between oxidant and antioxidant systems in favor of the former. ? 2014 Ahmadvand et al.; licensee BioMed Central Of different diabetic complications [6]. Several studies have shown that antioxidant treatment reduces diabetic complications [7]. Because of increasing demand of patients for the use of natural products and other herbal drugs with anti-diabetic activity, the general trend now is to use the natural products for medicinal application in their natural available form [8]. The research into the determination of the natural antioxidant source is very important to promote public health [10]
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