Ameliorating effects of rosemary and costus on blood-associated toxicity in Ehrlich-bearing mice treated with cisplatin

Abstract

Background: Rosemary (ROLE) and costus (SLRE) have been established to show antioxidant effects. Aim: This study aimed to show the ameliorate effects of ROLE and SLRE to side-effects induced by Cisplatin (CIS) in tumor-bearing mice. Materials and Methods: The extracts of ROLE and SLRE were examined for their phytochemical activities. Mice were inoculated with tumor cells treated with CIS at days 3-7 and ROLE or SLRE at days 3-14. Mice were sacrificed on day 14 for CBC and T-cell analysis. Results: Phytochemical analysis revealed that both ROLE and SLRE showed similar antioxidant activities. Treatment with EAC bearing mice with CIS induced antitumor efficacy of about 90%. The treatment with ROLE or SLRE alone had no antitumor activity with CIS. However, Co-administration of ROLE or SLRE with CIS increased significantly antitumor efficacy of CIS. . Flow cytometric analysis showed that the number of CD4+ and CD8+ T cells decreased in EAC bearing mice after treatment with CIS. Treatment with both ROLE and SLRE improved the number of these cells. Conclusion: A combination of rosemary and costus can enhance the antitumor activity and lower the side effects of cisplatin, as well as enhances cell-mediated immunity.