Ameliorating effect of L-Ascorbate on protein and ascorbic acid content in different tissues of freshwater bivalve Lamellidens marginallis on exposure to lambda-cyalothrin.

Abstract

Abstract: Ascorbic acid is one of the important tool to indicate the alterations induced by chemicals and pollutants. Ascorbic acid, being important constituent in cellular metabolism, the interactions of the biomolecules gives proper idea of toxicant stress and its effect. In the present investigation the freshwater bivalve Lamellidens marginalis were exposed to chronic dose of lambda-cyalothrin (0.75 PPM LC50/10 values) alone and in combination with 50mg/L L-ascorbic acid for 21 days respectively. Percent protein and ascorbic acid contents in the mantle, foot, gills, digestive glands, gonad and whole soft body of bivalve, Lamellidens marginalis on lambda-cyalothrin intoxication and in combination with 50mg/L L-ascorbic acid were observed. Protein and ascorbic acid content in all soft body tissue of lambda-cyalothrin exposed bivalve, Lamellidens marginalis showed remarkable decrease as compared to control. The maximum protein and ascorbic acid content was observed in foot and lowest in digestive gland. Animal exposed to lambda-cyalothrin intoxication in combination with 50 mg/L of L-ascorbic acid showed considerable reduction in the depletion of protein and ascorbic acid levels. Fast recovery of percent protein and ascorbic acid contents was observed in presence of L-ascorbic acid than the recovery in the normal freshwater. This study indicates the protective and curative property of the L-ascorbic acid against lambda-cyalothrin induced damage.
 Key Words: Lamellidens marginalis, lambda-cyalothrin, protein and L-ascorbic acid