Ameliorated light conditions increase the P uptake capability of soybean in a relay-strip intercropping system by altering root morphology and physiology in the areas with low solar radiation

Abstract

Belowground interspecific facilitation and complementarity contribute to the phosphorus (P) uptake advantages in the cereal-legume intercropping system. However, the root morphological and physiological plasticity and, subsequently, the P uptake capability response to light conditions in intercropping systems remain unclear. Soybean was grown under two levels of P application rates in sole and intercropping systems (maize/soybean relay strip intercropping) from 2016 to 2018 in Renshou, southwest of China. As a supplement to the field experiment, soybean was also grown in L-S (simulating the light conditions of sole cropping in the field: light first and then shading) and S-L (simulating the light conditions of intercropping in the field: shading first and then light) light conditions with two levels of P application in 2018 in a pot experiment. After maize harvest (approximately 3/4 of the soybean growth period), light capture in intercropping was higher than sole (ameliorated light conditions in intercropping system), which resulted in an advantage of P uptake in intercropped soybean. Both low P supply and more light capture increased the total root length and root APase activity. The genes GmEXPB2 (which is associated with root growth) and GmACP1 (which is associated with exudation of APase) were highly expressed in plants that captured more light under both P-sufficient and P-deficient conditions. Additionally, more light capture increased the production of lateral roots and the proportion of in the upper 15 cm soil layer roots at the reproductive stage in the field. Across the field and pot experiments, increased root morphological and physiological plasticity were associated with lower P concentrations in the leaves and greater allocation of photosynthates to roots as sucrose. It is suggested that ameliorated light conditions can regulate soybean root growth plasticity and, consequently, P uptake in maize/soybean relay strip intercropping systems, especially in the areas with low solar radiation.