Abstract
The following study aimed to investigate the hepato and neuro protective efficacy of Lycopine against Cisplatin which induced hepatotoxicity and neurotoxicity. Twenty Five male Wister rats were used for this experiment they were equally divided into 5 groups (5 rats per group): group (1) served as control group they were injected 1ml saline orally once daily for 20 day, group (2) served as Corn Oil group and they were administrated 1 mL Corn Oil orally once daily for 20 days, group (3) served as Lycopine group and they were administrated (10 mg/kg b.wt) Lycopine orally once daily for 20 days. , group (4) served as Cisplatin treated group and they were injected (6 mg/kg b.wt.) intrapertonialy once at day 10 of experiment and group (5) Lycopine+Cisplatin group and were administrated 10 mg/kg b.wt Lycopine orally once daily for 20 days and injected 6 mg/kg b.wt.) intraperitonialy once at day 10 . Result revealed that Cisplatin induced liver damage indicated by significant increase in liver biomarkers ALP, AlT, AST along with significant decrease in albumin, Moreover marked increase increase in tissue concentrations of malondialdehyde(MDA) and Total antioxidant(TAC) and reduce tissue Glutathione reductase(GSH),that indicated oxidative stress Also results revealed up regulation IL-6 and down regulation IL-10 in liver and brain tissue in compared to control group . However, interestingly concurrent adminsteration of the Lycopine orally at dose level of 10mg/kg b.wt for 20 days with Cisplatin can mitigate these toxic effects caused by Cisplatin.So it is concluded that the antioxidant and the anti-inflammatory effects of Lycopine moderate the Cisplatin-induced hepato and neurotoxicity.
Highlights
Results revealed that intrapertonial injection of Cisplatin at dose of 6 mg/kgb.wt once at day 10 showed significantly increased in ALP, ALTand AST by compared to control and corn oil group as well as significant decrease in albumin level,while interestingly it was found that concurrent adminsteration of Lycopine with Cisplatin result in significant in their levels ., albumin return to normal level . all these data illustrated in table (1)
It was found that the expression of IL6 significantly increased in liver and brain tissue by Cisplatin than control and corn oil group and by the action of Lycopine genes expression regained to normal levels
IL10 down regulated in liver and brain tissue of Cisplatin group than what’s happen in control group and corn oil group,while beneficially concurrent adminsteration of Lycopine with Cisplatin leads to upregulation of genes expression again Table (3),(4)
Summary
It kills cancer cells via various modes of actions beginning from oxidative stress, reactive oxygen species production, lipid peroxidation and activation of pro-inflammatory cytokines. These mechanisms of action may be an important way in the strategy of prevention of its side effects on normal cells Faten et al 2021, On other way it as a useful and effective drug in the treatment of many solid tumors, in other side it has many hazard effects including hepatotoxicity; sever kidney injury, ototoxicity and cardiac toxicity Gaskell et al .2018. Oxidative stress resulting from ROS may have a major role among these factors Veiga et al 2020
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