Ambient Air Pollution Exposure, Biomonitoring and Birth Weight: an Approach Combining Bioaccumulation of Trace Elements in Leaves and Tradescantia Micronucleus (Trad- MCN) Assay

Abstract

Ambient Air Pollution Exposure, Biomonitoring and Birth Weight: an Approach Combining Bioaccumulation of Trace Elements in Leaves and Tradescantia Micronucleus (Trad- MCN) AssayAbstract Number:2573 Camila M. Villegas-Cintra*, Ligia V. Barrozo, Alfésio L. F. Braga, Regiani Carvalho-Oliveira, National Institute for Integrated Analysi, Armando R. Tavares, Jorge L. M. Young, Shoey Kanashiro, Luiz A. A. Pereira, and Paulo H. N. Saldiva Camila M. Villegas-Cintra* Environmental Epidemiology Study Group, Laboratory of Experimental Air Pollution, Department of Pathology, Faculdade de Medicina da Universidade de São Paulo, Brazil, E-mail Address: [email protected] Search for more papers by this author , Ligia V. Barrozo School of Philosophy, Letters and Human Sciences, University of São Paulo, Brazil, E-mail Address: [email protected] Search for more papers by this author , Alfésio L. F. Braga Environmental Epidemiology Study Group, Laboratory of Experimental Air Pollution, Department of Pathology, Faculdade de Medicina da Universidade de São Paulo Universidade Católica de Santos, Brazil, E-mail Address: [email protected] Search for more papers by this author , Regiani Carvalho-Oliveira Environmental Epidemiology Study Group, Laboratory of Experimental Air Pollution, Department of Pathology, Faculdade de Medicina da Universidade de São Paulo Search for more papers by this author , National Institute for Integrated Analysi Brazil, E-mail Address: [email protected] Search for more papers by this author , Armando R. Tavares Botanical Institute, São Paulo, Brazil, Brazil, E-mail Address: [email protected] Search for more papers by this author , Jorge L. M. Young Botanical Institute, São Paulo, Brazil, Brazil, E-mail Address: [email protected] Search for more papers by this author , Shoey Kanashiro Botanical Institute, São Paulo, Brazil, Brazil, E-mail Address: [email protected] Search for more papers by this author , Luiz A. A. Pereira Environmental Epidemiology Study Group, Laboratory of Experimental Air Pollution, Department of Pathology, Faculdade de Medicina da Universidade de São Paulo Universidade Católica de Santos, Brazil, E-mail Address: [email protected] Search for more papers by this author , and Paulo H. N. Saldiva Environmental Epidemiology Study Group, Laboratory of Experimental Air Pollution, Department of Pathology, Faculdade de Medicina da Universidade de São Paulo, Brazil, E-mail Address: [email protected] Search for more papers by this author AbstractIntroduction: Air pollution has been associated to low birth weight (LBW), mainly in metropolitan areas. This study evaluated the response of Tradescantia pallida plants to air pollution in indoor and outdoor environments of residences with pregnants correlating it to newborn's birth weight (BW).Methods: The plants were exposed inside and outside of 39 residences in São Paulo city (Brazil) between 2012 and 2013. During pregnancies, monthly samples of T. pallida inflorescences and leaves were made. The inflorescences were analyzed by Trad-MCN assay and trace elements content in leaves was measured using an energy dispersive X Ray fluorescence spectrometer. Indoor, outdoor, and individual NO2 were measured with passive samplers and indoor PM2,5 were measured with active samplers. The residential addresses were geocoded using ArcGis (10.0). The municipality provided the digital cartographic bases, containing the classification of freeways and the traffic flow. We plotted buffers around to the nearest road for mothers’ addresses. The data for birth weight, accumulation of trace elements in leaves, average percentage values of micronucleus (Trad-MCN) and pollutants were represented in contour maps using Surfer (8.0).Results: The highest concentration point of micronuclei coincides with the areas of higher prevalence of LBW and high concentrations of PM2,5. The NO2 concentrations and the factor analysis for trace elements in leaves showed association with vehicular density.Conclusion: T. pallida is a good indicator of air pollutants, an useful for epidemiological studies..