盐胁迫下AM真菌对沙枣苗木光合与叶绿素荧光特性的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 盐胁迫下AM真菌对沙枣苗木光合与叶绿素荧光特性的影响 DOI: 10.5846/stxb201701030011 作者: 作者单位: 黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院;黑龙江大学生命科学学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金面上项目（31570635）；黑龙江省杰出青年科学基金（JC201306）；国家林业公益性行业科研专项经费项目（201504409）；哈尔滨市留学回国创业人才项目（2017RALXJ008） Effects of Arbuscular mycorrhizal fungi on photosynthetic and chlorophyll fluorescence characteristics in Elaeagnus angustifolia seedlings under salt stress Author: Affiliation: College of Life Sciences,Heilongjiang University,College of Life Sciences,Heilongjiang University,College of Life Sciences,Heilongjiang University,College of Life Sciences,Heilongjiang University;China;College of Life Sciences,Heilongjiang University;China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了揭示盐胁迫下AM真菌对苗木光合生理特性的影响，试验采用盆栽法，对接种AM真菌根内球囊霉（Glomus intraradices，GI）与未接种AM真菌（CK）的沙枣幼苗进行浓度为0、100、200、300mmol/L NaCl处理，测定不同处理沙枣苗木叶片的净光合速率Pn、气体交换参数（蒸腾速率Tr，气孔导度Gs，胞间二氧化碳Ci）、色素含量（叶绿素a、b，叶绿素，类胡萝卜素）、叶绿素荧光参数（最大荧光效率Fv/Fm，光系统Ⅱ效率ФPS Ⅱ，光化学淬灭系数qP，非光化学淬灭系数NPQ，表观电子传递速率ETR，光反应中心PSⅡ潜在活性Fv/Fo，热耗散速率HDR）等指标。结果表明：（1）随着盐浓度的增加，GI和CK处理对沙枣幼苗叶片Pn、Tr、Gs及Ci影响的变化趋势基本一致，均显著下降，但是在同一个盐浓度下，接种GI沙枣叶片的这些指标显著高于CK处理组（P<0.05），并且与不加盐处理为对照，其各参数的变化幅度显著低于CK组。（2）接种GI组和CK组的沙枣幼苗叶片随着盐浓度的增加色素含量各参数变化趋势基本一致，均降低或升高，但是与不加盐处理相比，CK处理组的变化幅度显著高于GI处理。（3）随着各处理盐浓度增加，接种GI处理的Fv/Fm、ФPS Ⅱ、qP、ETR、Fv/Fo呈先升高后下降的趋势，NPQ、HDR呈先降低后升高的趋势，相对应的CK处理组各值呈显著下降的趋势，而NPQ和HDR则呈先降低后升高以及逐渐升高的趋势，与不加盐处理为对照，GI处理组的变化幅度显著低于CK组。研究结果进一步揭示了AM真菌在盐生境中通过提高植物的光合和叶绿素荧光特性发挥重要的作用，而盐胁迫强度也是AM真菌发挥这一作用的影响因素。盐生植物与AM真菌共生用于盐碱地的改良具有一定的应用前景。 Abstract:The net photosynthetic rate (Pn), gas exchange parameters, chlorophyll fluorescence parameters, and chlorophyll content (chlorophyll a and b, and carotenoids) of Elaeagnus angustifolia leaves inoculated with arbuscular mycorrhizal fungi (GI) and non-inoculated treatments (CK) were measured during stress imposed by concentrations of 0, 100, 200, or 300 mmol/L NaCl. Gas exchange parameters included transpiration rate (Tr), stomatal conductance (Gs), and intercellular CO2 concentration (Ci). Chlorophyll fluorescence parameters included maximum fluorescence efficiency (Fv/Fm), actual photosystem Ⅱ efficiency (ФPSⅡ), photochemical quenching coefficient (qP), non-photochemical quenching coefficient (NPQ), apparent electron transfer rate (ETR), light reaction center of PS Ⅱ potential activity (Fv/Fo), and heat dissipation rate (HDR). The primary findings of these results were threefold. First, the Pn, Tr, Gs, and Ci of E. angustifolia leaves decreased significantly as salt concentration increased in both CK and GI treatments. Moreover, these parameters were significantly higher in GI treatments than in CK treatments (P<0.05). However, the magnitude of change in these parameters was smaller in GI treatments than in the CK treatment. Second, changes in chlorophyll content in E. angustifolia leaves as salt concentration increased were positively correlated in GI and CK treatments. However, the magnitude of such changes was generally higher in the CK treatments than in the GI treatments. Finally, in the GI treatments, Fv/Fm, ФPS Ⅱ, qP, ETR, and Fv/Fo initially increased with increasing salt concentration, before decreasing; in CK treatments, these parameters declined consistently as salt concentration increased. Moreover, in GI treatments, NPQ and HDR initially decreased as salt concentration increased, before increasing. In CK treatments, NPQ was similar to that of GI treatments, whereas HDR decreased gradually as the concentration of salt increased. The magnitude of effect across different salt concentrations in these parameters was significantly lower in GI treatments than in CK treatments. Inoculation with arbuscular mycorrhizal fungi (AMF) may promote salt tolerance in plants by improving photosynthetic and chlorophyll fluorescence characteristics, which in turn, promotes plant growth in saline habitats. We suggest that inoculation of halophytes with mycorrhizal symbionts can have potential applications in improving the usability of saline-alkali land. 参考文献 相似文献 引证文献