Alveolar ridge preservation with fibroblast growth factor-2 modified acellular dermal matrix membrane and a bovine-derived xenograft: An experimental in vivo study.

Abstract

To investigate the effect of a bone substitute material combined with fibroblast growth factor-2 (FGF-2) loaded barrier membrane on the preservation of alveolar ridge after tooth extraction. Four dogs were included. Six extraction sockets of each animal received the 3 treatments and were randomly divided into three groups. Group A: negative control; Group B: bovine xenografts+membrane; and Group C: bovine xenografts+FGF-2-loaded membrane. CBCT and histological analysis were performed to evaluate changes in the width and height of alveolar ridges and extraction socket bone healing 8weeks post-extraction. CBCT showed that the alveolar bone in Group A was significantly thinner than that in Group B and Group C at 1 and 3mm apically from the alveolar crest. The alveolar width at 1mm in Group C (60.99±15.36%) was significantly thicker than that in Group B (39.75±30.18%). Histomorphmetrical measurements showed that the buccal alveolar width at 1mm was significantly thicker in Groups B and C than in Group A. Additionally, buccal bone height and lingual bone width at 1mm in Group C (87.06±10.34%, 89.09±10.56%) were significantly greater than in Group A (53.48±23.94%, 82.72±12.59%). The present findings indicate that application of bovine bone combined with barrier membrane with or without FGF-2 over tooth sockets can effectively reduce ridge absorption, especially in terms of ridge width and FGF-2 modified membrane seems to improve the outcomes obtained with membrane alone.