Aluminum uptake and aluminum-induced rapid root growth inhibition of rice seedlings

Abstract

Aluminum (Al) inhibits root growth in acidic soil, but the site of action of Al remains unclear. We investigated whether the rate of Al accumulation correlates to Al-indeced rapid root growth inhibition in rice seedlings (Oryza sativa L. cv. Youngnam). Growth of roots was significantly inhibited by 100 μM AICI3, as early as 1 h after the treatment. The inhibition of root growth was strongly dependent on Al concentration (l50 = 20 (μM) and Al-exposure time (l50 = 23 min at 25 μM Al) in a solution of 10 mM KCI and 1 mM CaCl2 buffered by 10 mM Mes/KOH (pH 4.5). Using ICPES, massive uptake of Al by roots was observed even at 15 min treatment of 25 μM Al. The kinetics of Al uptake by the roots closely corresponded to the inhibitory effects of Al on root growth. When the roots of seedlings were exposed to 50 (μM Al for 1 h, then sectioned and stained with hematoxylin, all cell types of the roots showed the presence of Al in the cytoplasm. These results indicate that Al was rapidly taken up into the root cells and thereby reduced root growth.