Aluminum regulates oxalate secretion and plasma membrane H+-ATPase activity independently in tomato roots

Abstract

We demonstrated that aluminum (Al)-induced oxalate secretion and plasma membrane (PM) H(+)-ATPase activity in tomato (Lycopersicon esculentum 'Hezuo903') roots were poorly correlated. In addition, vanadate, an inhibitor of PM H(+)-ATPase, had no effect on Al-induced oxalate secretion, but significantly inhibited enzyme activity. An anion channel inhibitor phenylglyoxal inhibited oxalate secretion, but not PM H(+)-ATPase activity. Exposure of tomato roots to 10μM LaCl(3) also stimulated PM H(+)-ATPase activity; however, La failed to induce oxalate secretion. Furthermore, Al-induced changes of PM H(+)-ATPase activity were not associated with oxalate secretion in two tomato cultivars differing in the ability to secrete oxalate under Al stress. These results indicate that Al independently regulates oxalate secretion and PM H(+)-ATPase activity in tomato roots. Analysis of expression levels of PM H(+)-ATPase genes by real-time reverse transcription-PCR and protein by Western blot and immunodetection revealed that the regulation of PM H(+)-ATPase in response to Al was subjected to transcriptional and posttranscriptional control. However, since neither transcriptional level of genes nor translational level of proteins directly relate to the enzyme activity, posttranslational modification of PM H(+)-ATPase under Al stress likely contributes to changes in activity of this protein.