Alternative protein sources in diets for Japanese flounder Paralichthys olivaceus (Temminck and Schlegel): II. Effects on nutrient digestibility and digestive enzyme activity

Abstract

An 8-week feeding trial was conducted to compare the effects of dietary protein sources on nutrient digestibility and digestive enzyme activity. Four experimental diets were formulated to contain one of the following as the sole protein source: fish meal (FM), fish protein concentrate (FPC), soy protein concentrate (SPC) and soy protein isolate (SPI). Each diet was randomly assigned to triplicate aquaria stocked with 25 Japanese flounder (Paralichthys olivaceus) each. The dry matter, crude protein and energy digestibility and individual amino acid availability of the SPC-based diet were significantly lower than those of the other diets. The crude lipid digestibility of soy protein-based diets was significantly lower than that of the FM-based diet. The pepsin/protease activity was significantly higher in fish fed fish protein-based diets compared with fish fed soy protein-based diets. The lipase activity in fish fed the SPI-based diet was the highest among the dietary treatments. These results indicate that P. olivaceus can effectively digest the protein from FPC and SPI (but not SPC) as well as FM. The low protein digestibility and amino acid availability of the SPC-based diet may be related to the non-protein compounds present in SPC, whereas the low-lipid digestibility of soy protein-based diets may contribute to the undigested soy protein fractions and/or the alcohol-soluble components.