Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

Rafaela Barbosa Pares,Ana Paula Mamprim,Andreia Kusumota Bonini,Luis Francisco Angeli Alves

doi:10.1590/1808-1657000642015

Rafaela Barbosa Pares, Ana Paula Mamprim + Show 2 more

Open Access

https://doi.org/10.1590/1808-1657000642015

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Abstract

ABSTRACT: This work aimed to evaluate the effects of the products Biogermex®, Agro-mos®, Soil-Set® and Planta Clean® on the development of white muscardine in Bombyx mori. Products have been tested at two stages for effects on biology of caterpillars and on silk production (1), and then they were evaluated to know their potential in the prevention/control of white muscardine (2). Products were sprayed on mulberry leaves (preventive treatment) and also on the integument of silkworms (curative treatment). At the first stage, the products were applied on four consecutive days, in one of the daily feeds. Planta Clean® - curative treatment - and Soil-Set® - preventive treatment - caused mortality in 63.3% and 30% of silk worms, respectively. The fungus Beauveria bassiana was inoculated at the concentration 5 × 107 conidia/mL on the leaves and insects. The products were applied three hours after application on the fungus, in a single application, and also for four days in the other groups. A group of silkworms sprayed only with the fungus served as control. Biogermex® - curative treatment applied once and for four times - was the most effective, reducing mortality by 25 and 26.7%, respectively, as compared with the control group. None of the evaluated products acts preventively against white muscardine.

Highlights

Sericulture is an agro-based activity that employs relatively simple and low-tech equipment and facilities
The main fungal disease associated with Bombyx mori, is caused by the fungus Beauveria bassiana Bals. (Vuill.), especially in the first instars, and leads to larval death about six days after infection (AMARAL; ALVES, 1979; HANADA; WATANABE, 1986; KUMAR et al, 1999)
RAJAGOPAL et al (2014) observed the eficacy of a disinfectant based on chlorothalonil 1.6% (Kavach®) to prevent white muscardine in B. mori larvae

Summary

Introduction

Sericulture is an agro-based activity that employs relatively simple and low-tech equipment and facilities. (Vuill.), especially in the first instars, and leads to larval death about six days after infection (AMARAL; ALVES, 1979; HANADA; WATANABE, 1986; KUMAR et al, 1999) Preventive measures, such as disinfecting the premises and equipment, are taken before and after each silkworm batch is removed, followed by the spraying of a 3% formaldehyde solution (HANADA; WATANABE, 1986; ZANETTI, 2011). It should be noted that, even in the absence of silkworms, and despite all measures of disinfection of the premises and equipment, there is still contamination by microorganisms, in lower indices, as claimed by MARGATHO et al (2012) In this way, PARES; ALVES (2016) reviewed some alternatives to control silkworm white muscardine in B. mori silkworms. Insects infected and treated with the product showed more than 60% survival

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