Alternative Media from Mangrove Fruit for the Growth of Shigella dysentery, Escherichia coli, and Salmonella typhi bacteria

Abstract

Objectives: Mangroves are rich in bioactive substances that support bacterial growth. The fruit of the mangrove tree can serve as a valuable source of carbohydrates, making it a potential substitute for Nutrient Agar (NA) as a growth medium. Methodology and Results: The samples in this study were suspensions of Shigella dysentery, Escherichia coli, and Salmonella typhiwith 16 replications. Thus, the total number of samples in this study was 94 samples. The samples were isolated and identified as colony growth on alternative Mangrove and NA media. The results showed that in alternative media prepared from Mangrove fruit flour, there was a significant growth difference (p-value <0,01) between S. dysentery bacteria with an average of 61 colonies, E. coli bacteria with an average of 2 colonies, and S. typhi bacteria with an average of 2 colonies. The comparison between media of Mangrove and NA also showed a significant growth difference with p-value < 0,01. Conclusions: Mangrove media has been found to be less conducive to the growth of S. dysentery, E. coli, and S. typhi bacteria, despite being a more cost-effective option compared to NA. Notably, S. dysenteriae bacteria exhibit greater potential for propagation in this medium. Keywords: Alternative, culture, mangrove, media, nutrient agar