Abstract
YELLOW STRIPE-LIKE1 (YSL1) and YSL3 are involved in iron (Fe) and copper (Cu) translocation. Previously, we reported that upregulation of YSL1 and YSL3 under excess Cu caused high accumulation of Cu in the siz1 mutant, impaired in small ubiquitin-like modifier (SUMO) E3 ligase. Interestingly, the siz1 mutant contains high levels of salicylic acid (SA), involved in plant defense against biotrophic pathogens. In this study, we found that YSL1 and YSL3 were upregulated by SA. SA-regulated YSL3 but not YSL1 depended on NONEXPRESSOR OF PR1 (NPR1). Susceptibility to the pathogen Pseudomonas syringe pv. tomato (Pst) DC3000 was greater for ysl3 than the wild type. Also, during Pst DC3000 infection, YSL3 was positively regulated by SA signaling through NPR1 and the upregulation was enhanced in the coi1 mutant that defective in the jasmonic acid (JA) receptor, CORONATINE INSENSITIVE1. This line of evidence indicates that the regulation of YSL3 is downstream of SA signaling and interplays with JA signaling for involvement in pathogen-induced defense. We provide new insights into the biological function of the metal transporter YSL3 in plant pathogen defense.
Highlights
Plant species have 2 classes of mechanisms for acquiring iron (Fe): Strategy I is used by nongraminaceous plants to reduce ferric chelates at the root surface and absorb the generated ferrous ions across the root plasma membrane by iron transporters; strategy II is a chelation strategy used by graminaceous plants for primary acquisition of Fe [1]
The YELLOW STRIPE-LIKE (YSL) family in Arabidopsis was identified by sequence similarity to maize YELLOW STRIPE1 (YS1) (ZmYS1), which takes up ferric Fe–PS complexes and belongs to the oligopeptide transporter family [7,10,11]
We used wild-type plants treated with salicylic acid (SA) to investigate whether the expression of YELLOW STRIPE-LIKE1 (YSL1) and YSL3 is regulated by SA
Summary
Plant species have 2 classes of mechanisms for acquiring iron (Fe): Strategy I is used by nongraminaceous plants to reduce ferric chelates at the root surface and absorb the generated ferrous ions across the root plasma membrane by iron transporters; strategy II is a chelation strategy used by graminaceous plants for primary acquisition of Fe [1]. We used wild-type plants treated with SA to investigate whether the expression of YSL1 and YSL3 is regulated by SA. SA-induced YSL3 expression was diminished in the npr1 mutant, so the induction depended on NPR1 (Figure 2). SA only slightly induced YSL1 expression, with no NPR1 dependence (Figure 2).
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