Abstract
To present an alternative experimental model of third degree burn of easy reproducibility. Eighteen male Wister rats were randomly divided into three groups, 6 of which were allocated to each group. A soldering iron coupled to an aluminum plate was used to produce burn, at a temperature of 150ºC, with different exposure times per group. Group 5 (G5) animals were burned at 150°C with exposure time of 5 seconds; Group 10 (G10) the animals were burned at 150°C with exposure time of 10 seconds and group 15 (G15) the animals were burned at 150°C with exposure time of 15 seconds. Histopathological analyzes showed that all three groups had similar morphological characteristics, with total thickness involvement. The technique is effective to reproduce a third degree burn and suggests the temperature of 150ºC with 5 seconds of exposure in order to minimize the risks to the animals.
Highlights
Burns are tissue lesions that represent a severe form of trauma[1]
Several works using aluminum coupled to the soldering iron have been presented as an effective method for the reproduction of the experimental model of third degree burns[8,9,16-19], but there is a great discrepancy in the temperature used, which may vary from 100oC to 200oC, in addition to the time of application that varies between 5 to 30 seconds
Brassolatti et al.[18] presented a study in which the experimental model of third degree burn was carried out through an aluminum plate coupled to a soldering iron, but the temperature used was significantly lower, being 150oC with application on the back of the animal for 10 seconds, demonstrating that it is not necessary to use very high temperatures to perform this type of injury
Summary
Burns are tissue lesions that represent a severe form of trauma[1]. According to the World Health Organization, about 1.000,000 people are affected by burns each year in Brazil, and death records worldwide are close to 300,0002,3. In order to better explore the pathophysiology of burn and the healing process evolution through new treatment methods, the experimental model has been extensively used[5]. The current literature describes several protocols to perform burn in an experimental model, such as those performed with soldering iron, but there are differences related to both the size of the lesion, temperature and time of exposure. It is observed that the protocols are not yet well established for each degree of impairment to be induced, as some stipulate similar parameters for different degrees of burns[5-7]
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