Abstract

The genus Kalanchoe has become popular in the USA in recent years with many succulent species and cultivars available. Kalanchoe Crassulaceae family is native to Madagascar and tropical Africa. Kalanchoe plants with many small leaf spots were submitted to the Florida Extension Plant Diagnostic Clinic in Homestead, FL, during March 2017. Individual spots were dark brown, circular to irregular and ranged from 1 to 4 mm in diam. Surface-disinfected tissue was plated on PDA, and light grey to dark grey colonies grew abundantly after 7 days at 25oC under dark conditions. Microscopic observations revealed pale-brown to dark-brown conidia that were obclavate, obpyriform or ovoid. They varied in length from 20 to 50 µm (average 32.3 µm ± 3.4) and a width from 10-15 µm (10.8 µm ± 1.0) with 3 to 6 transverse septa and 0 to 2 longitudinal septa per conidium. The fungus was identified as an Alternaria species based on morphology (Woudenberg, et al. 2013). Sequences of the complete ITS region (MF174852), the gene fra...

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