Abstract
A novel Gram-negative, aerobic, motile, lemon-yellow-colored, and non-spore-forming rod-shaped bacterium designated strain NZ-12BT was isolated in February 2021 from a sponge species (Crateromorpha) collected at the southern Kermadec Ridge, Pacific Ocean, New Zealand. Comparative 16S rRNA gene-based analyses indicated that strain NZ-12BT shared 98.58%, 96.44%, 96.23%, and 94.78% 16S rRNA sequence similarity to Alteriqipengyuania lutimaris S-5T, Qipengyuania pelagi UST081027-248T, Qipengyuania citreus RE35F/1T, and Alteriqipengyuania halimionae CPA5T, respectively. The major respiratory quinone was ubiquinone-10(Q-10). The polar lipid profile of NZ-12BT was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methyl-ethanolamine, phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol, one unknown polar lipid, three unknown phospholipids, and three unknown glycolipids. The major fatty acids of strain NZ-12BT were C18:1ω12t, C16:0, C17:1ω6c, and C14:02-OH. Carotenoids were present. Genome mining analysis revealed a biosynthetic gene cluster encoding for the terpene biosynthesis. Pairwise ANI and dDDH values of strain NZ-12BT and closely related phylogenetic neighbors were below the threshold values of 95% and 70%, respectively. The DNA G+C content was 65.4 mol% (by genome). Based on data obtained by a polyphasic approach, type strain NZ-12BT (=DSM 112810T = NCCB 100841T) represents a novel species of the genus Alteriqipengyuania, for which the name Alteriqipengyuania abyssalis sp. nov. is proposed.
Highlights
The development of new antibiotics that are both safe and effective against resistant microbes is critical in combating the growing issue of antibiotic resistance [1,2]
The isolate was shown to be closely related to Alteriqipengyuania lutimaris S-5T, a member of the family Erythrobacteraceae based on comparative 16S
The strain was isolated from a sponge sample obtained in February 2017 on the southern Kermadec ridge, Pacific Ocean (350◦ 60/N, 178◦ 85/W) about 1165 m beneath the ocean surface
Summary
The development of new antibiotics that are both safe and effective against resistant microbes is critical in combating the growing issue of antibiotic resistance [1,2]. During the course of the study aimed at the isolation of novel bioactive compounds from deep-sea sponge-associated bacteria, we encountered a strain designated NZ-12BT. The isolate was shown to be closely related to Alteriqipengyuania lutimaris S-5T , a member of the family Erythrobacteraceae based on comparative 16S rRNA gene analyses. The taxonomy of the family Erythrobacteraceae was recently revised using genome-based methods, resulting in the creation of 11 new genera within the family [4]. Creation of novel genera has resulted in the placement of Erythrobacter lutimaris into a novel genus Alteriqipengyuania. Genus Alteriqipengyuania, a member of the family. Identification of novel microbial species with potential industrial relevance from extreme environments provides a framework for the improvement of sequence databases and may lead to the identification of new biotechnologically relevant entities. The present work aimed to determine the taxonomic position of NZ-12BT using a polyphasic characterization approach, including the determinations of chemotaxonomic and other phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences, and genetic analyses of genome data
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