Abstract
Carboxylated polyacrylamide resins l (Bio-Rad Labs., Richmond, CA, U.S.A.) offer excellent resolution of protein mixtures but suffer from several operational disadvantages, e.g. variable bed volumes and flow-rates and difhculties with regeneration Sn situ. For these reasons Bio-Rad Labs. has ceased to manufacture CMacrylamide gels and is offering other materials such as CM Bio-Gel A which have better mechanical properties. In preparing our own carboxyl polyacryIamide resin we have found that a modification of the technique used to prepare the commercial resins’ offers improved resolution and more reproducible flow-rates. The details of this synthesis are reported here.
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