Abstract
The spinal origin of jaundice-induced altered peripheral nociceptive response poorly understood. In the current study, we aimed to first validate rats with bile duct ligation (BDL) as a jaundice model accompanied by altered peripheral nociceptive response, and then to analyze differential gene and lncRNA expression patterns in the lower thoracic spinal cord on different time courses after BDL operation by using high-throughput RNA sequencing. The differentially expressed genes (DEGs) identified using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, followed by clustering analysis, Gene Ontology analysis and pathway analysis. As a result, a total of 2033 lncRNAs were differentially expressed 28d after BDL, in which 1545 probe sets were up-regulated and 488 probe sets were down-regulated, whereas a total of 2800 mRNAs were differentially expressed, in which 1548 probe sets were up-regulated and 1252 probe sets were down-regulated. The RNAseq data of select mRNAs and lncRNAs was validated by RT-qPCR. 28d after BDL, the expressions of lncRNA NONRATT002335 and NONRATT018085 were significantly up-regulated whereas the expression of lncRNA NONRATT025415, NONRATT025388 and NONRATT025409 was significantly down-regulated. 14d after BDL, the expressions of lncRNA NONRATT002335 and NONRATT018085 were significantly up-regulated; the expression of lncRNA NONRATT025415, NONRATT025388 and NONRATT025409 was significantly down-regulated. In conclusion, the present study showed that jaundice accompanied with decreased peripheral nociception involved in the changes of gene and lncRNA expression profiles in spinal cord. These findings extend current understanding of spinal mechanism for obstructive jaundice accompanied by decreased peripheral nociception.
Highlights
Obstructive jaundice is a clinical syndrome related to bile cholestasis and is governed by complex signals [1,2,3]
The RNAseq data of select mRNAs and long noncoding RNAs (lncRNAs) was validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). 28d after bile duct ligation (BDL), the expressions of lncRNA NONRATT002335 and NONRATT018085 were significantly up-regulated whereas the expression of lncRNA NONRATT025415, NONRATT025388 and NONRATT025409 was significantly down-regulated. 14d after BDL, the expressions of lncRNA NONRATT002335 and NONRATT018085 were significantly up-regulated; the expression of lncRNA NONRATT025415, NONRATT025388 and NONRATT025409 was significantly downregulated
We found that the expressions of lncRNA NONRATT002335 and NONRATT018085 were significantly up-regulated underlying BDL group compared with control group, whereas the expression of lncRNA NONRATT025415, NONRATT025388 and
Summary
Obstructive jaundice is a clinical syndrome related to bile cholestasis and is governed by complex signals [1,2,3]. Though there are various studies in clinical disorders by obstructive jaundice, its differential gene and lncRNA expression pattern in the thoracic spinal cord remains unknown. The introduction of high-throughput RNA sequencing (RNA-seq), where thousands of differential genes and lncRNA expression patterns can be studied in parallel [21,22,23,24], permits a broad assessment for altered expression of differential gene and lncRNA in the lower thoracic spinal cord on different time courses of obstructive jaundice model. We performed a comprehensive transcriptome analysis in obstructive jaundice model using RNA-seq, and identified lncRNAs with differential expression. We compared the expression of some lncRNAs in thoracic spinal cord at different time points in obstructive jaundice model
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