Abstract
Sensitivity of cotton (Gossypium hirsutum L.) seedlings to 6 C chilling was measured by following the development of ribonuclease (RNase) activity in cotyledons during the first week of germination. Increases in RNase activity observed in control tissues were delayed during chilling. Development of RNase activity was most sensitive to chilling during the second day of germination.Both total and specific activities of RNase preparations were similarly affected by chilling. Alterations of protein synthesis were suggested by comparisons of free polyribosome profiles from control and chilled tissues.Chilled seedlings partially recovered RNase activity following return to warm temperature. Recovery was slowest in seedlings that were chilled during both the second and third days of germination.
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