ALTERED AGEING IN SCHIZOPHRENIA: META-ANALYSIS OF CELL DENSITY STUDIES IN SCHIZOPHRENIA INDICATES DIRECTION OF CHANGE DEPENDS ON AGE OF SUBJECTS

Abstract

Mesial temporal lobe epilepsy (MTLE) is the most prevalent form of epilepsy, characterized by recurrent complex partial seizures and hippocampal sclerosis. The pathophysiology underlying this disorder remains unidentified. While a loss of benzodiazepine binding sites is a key diagnostic feature of MTLE, experimental studies have shown enhanced inhibitory transmission and increased expression of GABAA-receptors, suggesting that compensatory mechanisms are operative in epileptic hippocampus. In the present study, changes in the expression and cellular distribution of major GABAA-receptor subunits were investigated in the hippocampus of pilocarpine-treated rats during the phase of spontaneous recurrent seizures. A uniform decrease in GABAA-receptor subunit-immunoreactivity was observed in regions of extensive neuronal death (i.e. CA1, CA3, hilus), whereas a prominent increase occurred in the dentate gyrus (DG). Most strikingly, the increase was largest for the α3- and α5-subunits, which are expressed at very low levels in the DG of control rats, suggesting the formation of novel GABAA-receptor subtypes in epileptic tissue. Furthermore, an extensive loss of interneurons expressing the α1-subunit, representing presumptive basket cells, was seen in the DG. These changes were very similar to those reported in a novel mouse model of MTLE, based on the unilateral injection of kainic acid into the dorsal hippocampus (Bouilleret et al., 1999). This indicates that the regulation of GABAA-receptor expression is related to chronic recurrent seizures, and is not due to the extrahippocampal neuronal damage affecting pilocarpine-treated rats. These results allow causal relationships in the induction and maintenance of chronic recurrent seizures to be distinguished. The loss of a critical number of interneurons in the DG is a possible cause of seizure initiation, whereas the long-lasting upregulation of GABAA-receptors in granule cells represents a compensatory response to seizure activity.