Abstract
BackgroundThe objective of this study was to observe alterations of serum uric acid (SUA) level and gut microbiota after Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model.MethodWe performed Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. Serum uric acid (UA), xanthine oxidase (XO) activity, IL-6, TNF-α and lipopolysaccharide (LPS) level changes, and 16S rDNA of gut microbiota were analyzed.ResultsAfter the surgery, the RYGB and SG procedures significantly reduced body weight, serum UA, IL-6, TNF-α and LPS levels, and XO activity. In addition, the RYGB and SG procedures altered the diversity and taxonomic composition of the gut microbiota. Compared with Sham group, RYGB and SG procedures were enriched in the abundance of phylum Verrucomicrobia and species Akkermansia muciniphila, while the species Escherichia coli was reduced.DiscussionWe here concluded that bariatric surgery-induced weight loss and resolution of inflammatory remarkers as well as changes of gut microbiota may be responsible for the reduced XO activity and SUA level. To have a better understanding of the underlying mechanism of UA metabolism following bariatric surgery, further research is needed.
Highlights
Hyperuricemia (HUA), an abnormality in uric acid (UA) metabolism, which results in the increased serum uric acid (SUA), has become an important issue worldwide and is linked to gout and metabolic diseases, including hypertension, cardiovascular disease, and diabetes [1,2,3]
At the endpoint of week 8 during the experimental process, fecal samples from the Rouxen-Y gastric bypass (RYGB), sleeve gastrectomy (SG), Sham, and Control groups analyzed by 16S rDNA sequencing using Illumina MiSeq showed that at the phylum levels, Firmicutes, Bacteriodetes, Verrucomicrobia, and Proteobacteria accounted for almost 90% of the gut
We investigated the changes of body weight, serum IL-6, tumor necrosis factor-α (TNF-α), LPS levels, and xanthine oxidase (XO) activity following bariatric surgery
Summary
Hyperuricemia (HUA), an abnormality in uric acid (UA) metabolism, which results in the increased serum uric acid (SUA), has become an important issue worldwide and is linked to gout and metabolic diseases, including hypertension, cardiovascular disease, and diabetes [1,2,3]. The management of hyperuricemia includes decreased production or increased excretion of UA Lifestyle management such as weight reduction and dietary modification has less significant SUA-lowering effects [4,5,6]. A recent study has reported in a hyperuricemic mouse model showing decreased amount of bifidobacteria and lactobacilli, while increased serum UA level, XO activity, and LPS level [14]. The objective of this study was to observe alterations of serum uric acid (SUA) level and gut microbiota after Rouxen-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) surgery in a hyperuricemic rat model. Serum uric acid (UA), xanthine oxidase (XO) activity, IL-6, TNF-α and lipopolysaccharide (LPS) level changes, and 16S rDNA of gut microbiota were analyzed. Results After the surgery, the RYGB and SG procedures significantly reduced body weight, serum UA, IL-6, TNF-α and LPS levels, and XO activity. Compared with Sham group, RYGB and SG procedures were enriched in the abundance of phylum Verrucomicrobia and species Akkermansia muciniphila, while the species Escherichia coli was reduced
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