Abstract
It has been suggested that the mitochondrial chimeric gene orfH79 is the cause for abortion of microspores in Honglian cytoplasmic male sterile rice, yet little is known regarding its mechanism of action. In this study, we used a mass spectrometry-based quantitative proteomics strategy to compare the mitochondrial proteome between the sterile line Yuetai A and its fertile near-isogenic line Yuetai B. We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line. Western blotting showed that ORFH79 protein and ATP1 protein, an F(1) sector component of complex V, are both associated with large protein complexes of similar size. Respiratory complex activity assays and transmission electron microscopy revealed functional and morphological defects in the mitochondria of Yuetai A when compared with Yuetai B. In addition, we identified one sex determination TASSELSEED2-like protein increased in Yuetai A, leading to the discovery of an aberrant variation of the jasmonic acid pathway during the development of microspores.
Highlights
Cytoplasmic male sterility (CMS) is associated with mitochondrial defects
It has been suggested that the mitochondrial chimeric gene orfH79 is the cause for abortion of microspores in Honglian cytoplasmic male sterile rice, yet little is known regarding its mechanism of action
We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line
Summary
Cytoplasmic male sterility (CMS) is associated with mitochondrial defects. Results: Reduction of assembled mitochondrial protein complexes and altered jasmonic acid pathways were observed in the sterile line. We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line. Proteomic Analysis of HL-type CMS Rice Mitochondria revealed the chimeric gene orfH79 located downstream of the atp gene in the sterile line (10 –12). Transgenic analysis showed that the ORFH79 protein is responsible for CMS in the sterile line [13] It remains unclear how the aberrant ORFH79 affects organization and function in mitochondria. Respiratory complex activity assays and transmission electron microscopy revealed functional and structural defects in the mitochondria of YtA when compared with YtB. Our quantitative proteomic analyses revealed that one sex determination TASSELSEED2like protein was up-regulated in YtA, leading to the discovery that an aberrant jasmonic acid pathway existed in CMS rice
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