Abstract
The microbial communities that inhabit the laryngeal mucosa build stable microenvironments and have the potential to influence the health of the human throat. However, the associations between the microbiota structure and laryngeal carcinoma remain uncertain. Here, we explored this question by comparing the laryngeal microbiota structure in laryngeal cancer patients with that in control subjects with vocal cord polyps through high-throughput pyrosequencing. Overall, the genera Streptococcus, Fusobacterium, and Prevotella were prevalent bacterial populations in the laryngeal niche. Tumor tissue samples and normal tissues adjacent to the tumor sites (NATs) were collected from 31 laryngeal cancer patients, and the bacterial communities in laryngeal cancer patients were compared with control samples from 32 subjects. A comparison of the laryngeal communities in the tumor tissues and the NATs showed higher α-diversity in cancer patients than in control subjects, and the relative abundances of seven bacterial genera differed among the three groups of samples. Furthermore, the relative abundances of ten bacterial genera in laryngeal cancer patients differed substantially from those in control subjects. These findings indicate that the laryngeal microbiota profiles are altered in laryngeal cancer patients, suggesting that a disturbance of the microbiota structure might be relevant to laryngeal cancer.
Highlights
The human microbiota can be considered an organ composed of mixed species with functions that enable the construction of a polymicrobial assemblage[1, 2]
By analyzing the 16S rRNA gene variable region 3 (V3), we compared the microbiota structures in the tumor tissues and the normal tissues adjacent to the tumor sites (NATs) from laryngeal squamous cell carcinoma (LSCC) patients and in control tissues from subjects with vocal cord polyps
In the study presented here, we examined the laryngeal microbiota patterns in laryngeal cancer patients and control subjects
Summary
The human microbiota can be considered an organ composed of mixed species with functions that enable the construction of a polymicrobial assemblage[1, 2]. The altered expression of B7-H1 might induce the onset of chronic inflammatory disease, which frequently precedes the development of human cancers[24]. This bacterium might induce proenzyme matrix metalloproteinase 9 (proMMP9) expression by activating the ERK1/2-Ets[1], p38/HSP27, and PAR2/NF-kB pathways[25], and the overexpression of proMMP9 is able to promote the cellular invasion ability of OSCC cells[25]. By analyzing the 16S rRNA gene variable region 3 (V3), we compared the microbiota structures in the tumor tissues and the normal tissues adjacent to the tumor sites (NATs) from laryngeal squamous cell carcinoma (LSCC) patients and in control tissues from subjects with vocal cord polyps
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