Alterations of Metabolic Profile in The Plasma of High-fat High-fructose Diet-induced Obese Rats

Abstract

Obesity, a kind of metabolic diseases, is tightly linked to many chronic diseases, such as cardiovascular diseases, non-alcohol fatty liver diseases and diabetes. However, the underlying molecular mechanisms of obesity remain uncertain. In order to comparatively analyze the plasma metabolome and present some clues for relative research on recovering the underlying molecular mechanisms, we performed a gas chromatography–mass spectrometry-based metabolic profiling of the plasma of obese rats induced by high-fat high-fructose diet. The changes of plasma metabolism between the diet-induced obese rats(n=8) and healthy control rats(n=7) were studied and the significant metabolites were identified by multivariate statistical analysis, including principal component analysis (PCA) and pair-wise orthogonal projections to latent structures discriminant analysis (OPLS-DA). Compared with control rats, diet-induced obese rats were characterized by 16 lower level metabolites and 23 higher level metabolites. These molecular changes were closely related to amino acid metabolism, carbohydrates metabolism and lipid metabolism. These findings may provide insight into the pathophysiological mechanisms underlying obesity and could be of valuable assistance in the early clinical diagnosis of obesity associated diseases.