Alterations of leaf characteristics and peroxidase activity in diploid androgenetic lines of Nicotiana sylvestris spegaz. et comes

Abstract

Two series of diploid androgenetic lines of Nicotiana sylvestris and their original line were investigated for leaf characteristics and peroxidase activity. The first series included two androgenetic lines (‘flat’ and ‘crumpled’ phenotypes named after the leaf aspect) derived from the same doubled haploid regenerated plant. Compared to the original line, the leaves were less developed, although cell size and peroxidase activity was higher. The second series comprised androgenetic lines obtained by consecutive cycles of androgenesis. These plants had smaller leaves, larger cells, and increasing peroxidase activity through consecutive cycles of androgenesis. The ‘crumpled’ phenotype was also more and more marked with each cycle. Results are discussed in relation to other analyses performed on these plants (inheritance, DNA analysis, DNA content).