Abstract

Scanning electron microscopy allowed us to distinguish the successive phases induced on the integument by virulent and nonvirulent strains. By chemical analyses, the epicuticular hydrocarbons alterations were observed on Ostrinia nubilalis and Melolontha melolontha larval integument. More precisely, only within the first 6 hr or so, even before the spore germination, several monomethylalkanes completely disappeared, even if the strain was not able to specifically penetrate in the host. Finally, between 32 and 51% of the cuticular hydrocarbons of M. melolontha and 77 and 86% of those of O. nubilalis might be affected 96 hr after a cuticular application of conidia of Beauveria bassiana or B. brongniartii.

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