Alterations of Binding Mode and Cutting Site by G→I Replacement in Preferred Cleavage Sequence 5′-Agg of Chromoprotein C-1027

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Replacement of guanosine by inosine at the center position of a target trinucleotide 5′-AGG resulted in greatly diminished DNA cleavage by C-1027 chromophore, indicating that the guanine 2-amino group significantly participates to DNA binding and cleavage by the chromophore of C-1027. This notion was supported by fluorescence titrations of the chromophore with [poly(dG-dC)] 2 and [poly(dI-dC)] 2. In preferred cleavage sequence 5′-AGG of the enediyne chromophore of C-1027, the G→I substitution clearly induces change of DNA binding mode and cutting site.