Alterations in The Plasma Expression of mir-15b, mir-195 and the Tumor-Suppressor Gene DLEU7 in Patients with B-Cell Chronic Lymphocytic Leukemia.

Behzad Khansarinejad,Alireza Moradabadi,Ghasem Mosayebi,Mahdieh Mondanizadeh,Malihe Bagheri

doi:10.52547/rbmb.10.1.20

Behzad Khansarinejad, Alireza Moradabadi + Show 3 more

Open Access

https://doi.org/10.52547/rbmb.10.1.20

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Abstract

Chronic lymphocytic leukemia (CLL) is one of the most prevalent forms of leukemia in adults. Inactivation of the DLEU7 gene is frequently observed in patients with CLL. Furthermore, microRNAs (miRNAs) have been observed to have a critical role in the pathogenesis of several cancers, including leukemia. Considering the tumor-suppressive role of DLEU7, as well as the tumor suppressor or oncogenic role of microRNAs (miRNAs), the aim of the present study was to evaluate the potential miRNAs targeting the DLEU7 gene in B-cells and explore expression changes these genes in the plasma of B-CLL patients. The miRNAs interacting with the DLEU7 gene were predicted and selected using bioinformatics tools. A total of 80 plasma samples were collected from 40 patients with B-cells and 40 healthy individuals, then subjected to RNA extraction and cDNA synthesis. The expression profiles of the predicted miRNAs and the DLEU7 gene in the plasma of B-CLL patients and healthy individuals were determined by RT-qPCR analysis. The bioinformatics prediction indicated that miR-15b and miR-195 target the DLEU7 gene. The expression levels of miR-15b and miR-195 were significantly higher in the plasma of patients with B-CLL compared to the healthy individuals (91.6, p= 0.001) (169, p= 0.001). However, the expression level of the DLEU7 gene was found to be significantly lower in the patient group compared to healthy controls (0.304, p= 0.001). Both miR-15b and miR-195, have the potential to function as novel and non-invasive biomarkers in the diagnosis and prognosis of patients with B-CLL.

Highlights

Chronic lymphocytic leukemia (CLL) is one of the most common forms of leukemia, primarily affecting the older adult population [1]
MiRNAs prediction The predictions of various bioinformatics databases such as PicTar, DIANA, Targetscan, miRDB, miRecords, and RNAhybrid revealed that miR-15b and miR-195 are the miRNAs that target the DLEU7 gene with the high repetition (Table 4)
Some of the databases, including Pictar, DIANA, Targetscan, and miRDB, reported the connection of miR-15b and miR-195 to the DLEU7 gene based on the indicators, free energy, miTG score, context score, and target score, respectively (Table 5), while miRecords and RNAhybrid only revealed connection or non-connection

Summary

Introduction

Chronic lymphocytic leukemia (CLL) is one of the most common forms of leukemia, primarily affecting the older adult population [1]. These abnormal B-cells accumulate in the blood, bone marrow, and secondary lymphoid tissues [3]. On their cell surface, they express CD5, CD19, CD23, and low levels of membrane immunoglobulins (IgM and IgD) [4]. The expression profiles of the predicted miRNAs and the DLEU7 gene in the plasma of B-CLL patients and healthy individuals were determined by RT-qPCR analysis. The expression levels of miR-15b and miR-195 were significantly higher in the plasma of patients with BCLL compared to the healthy individuals (91.6, p= 0.001) (169, p= 0.001). Conclusions: Both miR-15b and miR-195, have the potential to function as novel and non-invasive biomarkers in the diagnosis and prognosis of patients with B-CLL

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