Abstract
So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF2α and PGE2) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4–7 years), and old (8–14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF2α, and PGE2, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE2, and PGF2α were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE2, and PGF2α concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine.
Highlights
Equine endometritis has been considered as the most important cause of infertility in horses [1]
Previous studies reported that the expression of pro-inflammatory cytokines IL-6 and prostaglandins (PGF2α and PGE2) in the uterine tissue samples were associated with the development of endometritis in cows [9] and mares [6,8]
Nothing is known about the expression pattern of serum miRNA and post-transcriptional regulation of inflammatory immune response genes in mares with endometritis
Summary
Equine endometritis has been considered as the most important cause of infertility in horses [1]. Previous studies reported that the expression of pro-inflammatory cytokines IL-6 and prostaglandins (PGF2α and PGE2) in the uterine tissue samples were associated with the development of endometritis in cows [9] and mares [6,8]. Nothing is known about the expression pattern of serum miRNA and post-transcriptional regulation of inflammatory immune response genes in mares with endometritis. The miRNA are small non-coding RNA molecules that act as post-transcriptional regulators of gene expression by inhibiting translation or degrading mRNA through partial or complete base pairing with three prime untranslated region (3 -UTR) of the target mRNAs [17] They are expressed in different cells and tissues, in order to regulate different mechanisms of developmental as well as physiological processes [16,18]. The current study aimed (I) to investigate the expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205, and (II) to measure the concentrations of IL-6, PGF2α, and PGE2, in serum of young and old aged mares with healthy and abnormal uterine status (endometritis)
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