Abstract
Summary Determinations of the specific density of spermatozoa in ejaculates collected from normal dairy bulls in regular service were performed by centrifugation in umbradil-methylglucamine-salt solutions of different specific gravities. A negative correlation between the mean specific gravity of the spermatozoa and the frequencies of unripe spermatozoa (having a residual protoplasmic drop) was found, indicating a lower specific gravity in these than in ripe and over-ripe (non-motile) ones. The distribution of the three kinds of spermatozoa between the sedimenting and floating fractions of cells supports this conclusion, and suggests a higher specific density of over-ripe than of ripe spermatozoa. The process of ripening thus implies a continuous rise in density.
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