Abstract
Asthma often begins in childhood, although making an early diagnosis is difficult. Clinical manifestations, the exclusion of other causes of bronchial obstruction, and responsiveness to anti-inflammatory therapy are the main tool of diagnosis. However, novel, precise, and functional biochemical markers are needed in the differentiation of asthma phenotypes, endotypes, and creating personalized therapy. The aim of the study was to search for metabolomic-based asthma biomarkers among free amino acids (AAs). A wide panel of serum-free AAs in asthmatic children, covering both proteinogenic and non-proteinogenic AAs, were analyzed. The examination included two groups of individuals between 3 and 18 years old: asthmatic children and the control group consisted of children with neither asthma nor allergies. High-performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS technique) was used for AA measurements. The data were analyzed by applying uni- and multivariate statistical tests. The obtained results indicate the decreased serum concentration of taurine, L-valine, DL-β-aminoisobutyric acid, and increased levels of ƴ-amino-n-butyric acid and L-arginine in asthmatic children when compared to controls. The altered concentration of these AAs can testify to their role in the pathogenesis of childhood asthma. The authors’ results should contribute to the future introduction of new diagnostic markers into clinical practice.
Highlights
IntroductionIt is a chronic illness, caused by both genetic and eAnvsitrhomnmaeinstaalsfearcitoourss apnrdobclheamracintercioznedtebmyptohrearinyflmamedmiactiinoen
In the face of the high prevalence of asthma in society and the need for the rapid implementation of treatment, it is important to have quantifiable indicators that correlate with the development of
In the face of the high prevalence of asthma in society and the need for the rapid implementation of treatment, it is important to have quantifiable indicators that correlate with the development of the disease
Summary
It is a chronic illness, caused by both genetic and eAnvsitrhomnmaeinstaalsfearcitoourss apnrdobclheamracintercioznedtebmyptohrearinyflmamedmiactiinoen. OIft itshea lcohwroerniaciriwllanyess.s, caused by both Inflammgaetinonetliecadasndto eonedveirmoanmanedntthael ifnafcilttorarstioannodf tchheabrarocntecrhiizael dmubcyostah,ebrionnflcahmosmpaastmio,nanodf atnhe lower airways. ExcessiveInmflaumcums asetciorentiloena,drsestuoltoinegdeinmareavnerdsibthlee ainirfiwlatryatoibosntroufcttihone.bHroonwcehviearl, minuacossigan, ibfircoannct hospasm, and an paierrfcloewntaleigmxecioteafstpisoaivnti.eeTnmhtse,uhcchiugrhosenssiect ciinnrecflitadimoennmc,eartreiaostnuesllteoianfdagssttihonmthraeeavrreeemrrseoicbdoleerldinaegdirowinf tadhyeevboerbloosnptcrehudicactloiwounnat.lrlsieHasno[d1w]f.ieTxveheder, in a significant recent glpoebracleenptiadgeemoioflpogaitcieanl rtse,pcohrtrohnasicsihnoflwanmtmhaattnioonwlaedaadyss ttohitshaeilrmemenotdiselaifnfgecotifngthme obrreonthcahnial walls and fixed 300 milliaoinrflpoewoplleimaliltaotvieornt.heTwheorhldig. Ihnesmtainnycipdaetniecnetsr, aatsethsmoaf absetghinms ainacrheilrdehcooordde[d2].inRedcuervreelnotped countries [1]. WheezinTgheepriseocdeenst tgrliogbgearleedpibdyevmiriaolloingfieccatliornepaorret thhaesmshoostwcnomthmaot nnomwaandifaeystsattihoins aofilmasethnmt ias ianffecting more than 300 million people all over the world. Asthma begins in childhood [2]. Res. Public Health 2020, 17, x; doi: www.mdpi.com/journal/ijerph
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
